Application
Immunofluorescence of PRDM16 in Human Brain cells with PRDM16 antibody at 20 μg/mL.Application
Western blot analysis of PRDM16 in K562 cell lysate with PRDM16 antibody at 1 μg/mL.| Product Name | PRDM16 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | PRDM16 Antibody: PRDM16 is a zinc finger transcription factor and contains an N-terminal PR domain. The reciprocal translocation t(1;3)(p36;q21) occurs in a subset of myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). This gene is located near the 1p36.3 breakpoint and has been shown to be specifically expressed in the t(1:3)(p36, q21)-positive MDS/AML. The translocation results in the overexpression of a truncated version of this protein that lacks the PR domain, which may play an important role in the pathogenesis of MDS and AML. Recent studies have shown that PRDM16 normally acts as a Smad3 binding protein that may be important for the development of orofacial structures through modulation of the TGF-beta signaling pathway. Other experiments have indicated that PRDM16 controls a bidirectional cell fate switch between skeletal myoblasts and brown fat cells.1) Mochizuki N, Shimizu S, Nagasawa T, et al. A novel gene, MEL1, mapped to 1p36.3 is highly homologous to the MDS1/EVI1 gene and is transcriptionally activated in t(1;3)(p36;q21)-positive leukemia cells. Blood2000; 96:3209-14. |
| Immunogen | PRDM16 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human PRDM16. The immunogen is located within amino acids 1120 - 1170 of PRDM16. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| PRDM16 antibody can be used for detection of PRDM16 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRDM16 |
|---|---|
| Alternative Names | PRDM16 Antibody: MEL1 LVNC8 PFM13 CMD1LL KIAA1675 MEL1 PR domain zinc finger protein 16 PR domain-containing protein 16 |
| Molecular Weight(MW) | Predicted: 109, 120, 140 kDa Observed: 140 kDa |
Application
Immunofluorescence of PRDM16 in Human Brain cells with PRDM16 antibody at 20 μg/mL.
Application
Western blot analysis of PRDM16 in K562 cell lysate with PRDM16 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | PRDM16 antibody can be used for detection of PRDM16 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | PRDM16 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PRDM16 Antibody is supplied in PBS containing 0.02% sodium azide. |
