Application
Western blot analysis of PRDX3 using rabbit polyclonal PRDX3 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PRDX3 gene.Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PRDX3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Flow cytometric analysis of MDA-MB468 cells using PRDX3 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of PRDX3 Antibody in NCI-H460,MDA-MB231,HepG2 cell line lysates (35ug/lane)(2ug/ml)| Product Name | PRDX3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | PRDX3 is a protein with antioxidant function and is localized in the mitochondrion. This protein is involved in redox regulation of the cell and Protects radical-sensitive enzymes from oxidative damage by a radical-generating system.It Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.1) Cox,A.G., et.al., Biochemistry 48 (27), 6495-6501 (2009) |
| Immunogen | This PRDX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-94 amino acids from the N-terminal region of human PRDX3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | PRDX3 |
|---|---|
| Alternative Names | Thioredoxin-dependent peroxide reductase mitochondrial Antioxidant protein 1 AOP-1 HBC189 Peroxiredoxin III Prx-III Peroxiredoxin-3 Protein MER5 homolog PRDX3 AOP1 |
| Molecular Weight(MW) | 28 kDa |
Application
Western blot analysis of PRDX3 using rabbit polyclonal PRDX3 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PRDX3 gene.
Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PRDX3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Flow cytometric analysis of MDA-MB468 cells using PRDX3 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of PRDX3 Antibody in NCI-H460,MDA-MB231,HepG2 cell line lysates (35ug/lane)(2ug/ml)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
