Application
Immunohistochemical staining of rat brain tissue using precerebellin antibody at 2 μg/mL.Application
Western blot analysis of precerebellin in mouse cerebellum lysate with precerebellin antibody at (A) 2 and (B) 4 μg/mL.| Product Name | Precerebellin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Precerebellin Antibody: Cerebellin-1, Precerebellin, CER |
| Product description | Precerebellin Antibody: Precerebellin is the precursor of the brain-specific hexadecapeptide cerebellin, a protein with substantial similarity to the globular region of the B chain of complement component C1q. Cerebellin exerts neuromodulatory functions by directly stimulating norepinephrine release via the adenylate cyclase/pka- dependent signaling pathway; and indirectly enhances adrenocortical secretion in vivo, through a paracrine mechanism involving medullary catecholamine release. The active form of precerebellin is highly enriched in postsynaptic structures of cerebellar Purkinje cells in cartwheel neurons of the dorsal cochlear nucleus. Because precerebellin belongs to the C1q/tumor necrosis factor superfamily of secreted proteins and has similarity to adiponectin and CTRP3, it has been suggested that precerebellin posseses functions other than those already stated.1) Urade Y, Oberdick J, Molinar-Rode R, et al. Precerebellin is a cerebellum-specific protein with similarity to the globular component C1q B chain. Proc. Natl. Acad. Sci. USA1991; 88:1069-73. |
| Immunogen | Precerebellin antibody was raised against a 15 amino acid synthetic peptide from the middle of human precerebellin. The immunogen is located within amino acids 50 - 100 of Precerebellin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| Precerebellin antibody can be used for the detection of precerebellin by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CBLN1 |
|---|---|
| Alternative Names | Precerebellin Antibody: Cerebellin-1 Precerebellin CER |
| Sequence Similarities | 2) Cat. No. 1463 - Rat Brain Tissue Lysate |
Application
Immunohistochemical staining of rat brain tissue using precerebellin antibody at 2 μg/mL.
Application
Western blot analysis of precerebellin in mouse cerebellum lysate with precerebellin antibody at (A) 2 and (B) 4 μg/mL.| Positive Control | 1) Cat. No. 1412 - Mouse Cerebellum Tissue Lysate |
|---|---|
| Application Notes | Precerebellin antibody can be used for the detection of precerebellin by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Precerebellin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Precerebellin Antibody is supplied in PBS containing 0.02% sodium azide. |
