Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of anti- in Hela cell line lysates (35ug/lane)Application
Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the PRMT1 antibody detected the PRMT1 protein (arrow).| Product Name | PRMT1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protein arginine N-methyltransferase 1, 211-, Histone-arginine N-methyltransferase PRMT1, Interferon receptor 1-bound protein 4, PRMT1, HMT2, HRMT1L2, IR1B4 |
| Product description | Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins, IL enhancer binding factor, nuclear factors, cell cycle proteins, signal transduction proteins, apoptosis proteins, and viral proteins. The mammalian PRMT family currently consists of 7 members that share two large domains of homology.1) Zhang, X., et al., EMBO J. 19(14):3509-3519 (2000). |
| Immunogen | This PRMT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 317-346 amino acids from the C-terminal region of human PRMT1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | PRMT1 |
|---|---|
| Alternative Names | Protein arginine N-methyltransferase 1 211- Histone-arginine N-methyltransferase PRMT1 Interferon receptor 1-bound protein 4 PRMT1 HMT2 HRMT1L2 IR1B4 |
| Molecular Weight(MW) | 42 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of anti- in Hela cell line lysates (35ug/lane)
Application
Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the PRMT1 antibody detected the PRMT1 protein (arrow).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
