Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PRMT3 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of anti-PRMT3 Pab in whole HL60 cell lysate: PRMT3 was detected using purified Pab.Application
Western Blot at 1:2000 dilution + mouse brain lysates Lysates/proteins at 20 ug per lane.| Product Name | PRMT3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protein arginine N-methyltransferase 3, 211-, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3, PRMT3, HRMT1L3 |
| Product description | Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins, IL enhancer binding factor, nuclear factors, cell cycle proteins, signal transduction proteins, apoptosis proteins, and viral proteins. The mammalian PRMT family currently consists of 7 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all 7 PRMT members have been developed.1) Tang, J., et al., J. Biol. Chem. 273(27):16935-16945 (1998). |
| Immunogen | This PRMT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 142-173 amino acids from the N-terminal region of human PRMT3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | PRMT3 |
|---|---|
| Alternative Names | Protein arginine N-methyltransferase 3 211- Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3 PRMT3 HRMT1L3 |
| Molecular Weight(MW) | 60 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PRMT3 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of anti-PRMT3 Pab in whole HL60 cell lysate: PRMT3 was detected using purified Pab.
Application
Western Blot at 1:2000 dilution + mouse brain lysates Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
