Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Progesterone Receptor (Phospho-Ser190). Bottom Image: Immunofluorescence staining of methanol-fixed MCF cells using Progesterone Receptor (Phospho-Ser190).Application
Western blot analysis of lysed extracts from SKOV3 cells untreated (Lane 1) or treated with EGF (lane 2) using Progesterone Receptor (Phospho-Ser190).| Product Name | Progesterone Receptor (phospho Ser190) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PR, NR3C3, IL10R, PGR, PRGR |
| Product description | Progesterone receptors (PRs) are nuclear hormone receptors of the NR3C class, which also includes mineralocorticoid, glucocorticoid and androgen receptors. They exist as homodimers coupled to Hsp90 or HMGB proteins, which are shed upon activation. The major signaling pathway used by progesterone receptors is via direct DNA binding and transcriptional regulation of target genes. They can also signal by binding to other proteins, mainly with transcription factors such as NF-κB, AP-1 or STAT. Progesterone receptors are found in the female reproductive tract, mammary glands, brain and pituitary gland and receptor expression is induced by estrogen. Well established functions of progesterone receptors include ovulation, implantation, mammary gland development and maintenance of pregnancy. In addition, progesterone, signaling through the progesterone receptor, increases the ventilatory response of the respiratory centers to carbon dioxide and decreases arterial and alveolar PCO2 in the luteal phase of the menstrual cycle and during pregnancy. The human gene encoding the progesterone receptor has been localized to 11q22.1) Narayanan R, et al. (2005) Mol Cell Biol; 25 (8): 2885-98. Knotts TA, et al. (2001) J Biol Chem; 276 (11): 8475-83. Clemm DL, et al. (2000) Mol Endocrinol; 14 (1): 52-65. Zhang Y, et al. (1997) Mol Endocrinol; 11 (6): 823-32 |
| Immunogen | Progesterone Receptor (Phospho-Ser190) antibody was raised against a peptide sequence around phosphorylation site of serine 190 (G-L-S (p) -P-A) derived from Human Progesterone Receptor. |
| Modification | p-Ser190 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PGR |
|---|---|
| Alternative Names | PR NR3C3 IL10R PGR PRGR |
| Molecular Weight(MW) | 99 kDa |
| Tissue Specificity | This antibody detects endogenous level of Progesterone Receptor only when phosphorylated at serine 190. |
| Entrez Gene | 5241 |
|---|---|
| Protein Accession | NP_000917.3 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Progesterone Receptor (Phospho-Ser190). Bottom Image: Immunofluorescence staining of methanol-fixed MCF cells using Progesterone Receptor (Phospho-Ser190).
Application
Western blot analysis of lysed extracts from SKOV3 cells untreated (Lane 1) or treated with EGF (lane 2) using Progesterone Receptor (Phospho-Ser190).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
