Application
Western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the PHB1 antibody detected the PHB1 protein (arrow).Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with Prohibitin (PHB1) Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Flow cytometric analysis of ATDC5 cells using PHB1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | Prohibitin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Prohibitin, PHB |
| Product description | Prohibitin is an evolutionarily conserved protein that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region.1) Gregory-Bass,R.C., Int. J. Cancer 122 (9), 1923-1930 (2008) Ross,J.A., J. Biol. Chem. 283 (8), 4699-4713 (2008) White,J.J., Genomics 11 (1), 228-230 (1991) |
| Immunogen | This Prohibitin (PHB1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92-121 amino acids from the N-terminal region of human Prohibitin (PHB1). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PHB |
|---|---|
| Alternative Names | Prohibitin PHB |
| Molecular Weight(MW) | 30 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the PHB1 antibody detected the PHB1 protein (arrow).
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with Prohibitin (PHB1) Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Flow cytometric analysis of ATDC5 cells using PHB1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
