Application
Immunocytochemistry of PROM1 in Jurkat cells with PROM1 antibody at 2.5 μg/mL.Application
Immunofluorescence of PROM1 in Jurkat cells with PROM1 antibody at 20 μg/mL.Application
Western blot analysis of PROM1 in Jurkat cell lysate with PROM1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.IF
Immunofluorescence staining showed that the expression of stem cell markers in LAT1− and LAT1+ cells from U87 and U251 (A).| Product Name | PROM1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | PROM1 is a pentaspan transmembrane glycoprotein that localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation (1). Mutations in this gene have been shown to result in retinitis pigmentosa (2). Expression of this gene is also associated with several types of cancer (3).1) Yin AH, Miraglia S, Zanjani ED, et al. AC133, a novel marker for human hematopoietic stem and progenitor cells. Blood 1997; 90:5002-12. |
| Immunogen | PROM1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human PROM1. The immunogen is located within the last 50 amino acids of PROM1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| PROM1 antibody can be used for detection of PROM1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry at 2.5 μg/mL. For Immunoflorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PROM1 |
|---|---|
| Alternative Names | PROM1 Antibody: RP41 AC133 CD133 MCDR2 STGD4 CORD12 PROML1 MSTP061 Prominin-1 Antigen AC133 |
| Molecular Weight(MW) | Predicted: 95 kDa Observed: 97 kDa |
| Sequence Similarities | 2) Cat. No. 17-005 - Jurkat Cell Slide |
| Tissue Specificity | PROM1 antibody is human, mouse and rat reactive. Multiple isoforms of PROM1 are known to exist. |
Application
Immunocytochemistry of PROM1 in Jurkat cells with PROM1 antibody at 2.5 μg/mL.
Application
Immunofluorescence of PROM1 in Jurkat cells with PROM1 antibody at 20 μg/mL.
Application
Western blot analysis of PROM1 in Jurkat cell lysate with PROM1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
IF
Immunofluorescence staining showed that the expression of stem cell markers in LAT1− and LAT1+ cells from U87 and U251 (A).| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | PROM1 antibody can be used for detection of PROM1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry at 2.5 μg/mL. For Immunoflorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PROM1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | PROM1 Antibody is supplied in PBS containing 0.02% sodium azide. |
