Application
Immunohistochemistry of PPR5 in mouse brain tissue with PPR5 antibody at 5 μg/mL.Application
Immunofluorescence of PPR5 in mouse brain tissue with PPR5 antibody at 20 μg/mL.Application
Western blot analysis of PRR5 in SK-N-SH cell lysate with PRR5 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide| Product Name | PRR5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRR5 Antibody: PP610, PROTOR1, PROTOR-1, FLJ20185k, PP610, Proline-rich protein 5, Protein observed with Rictor-1, Protor-1 |
| Product description | PRR5 Antibody: Proline-rich protein 5 (PRR5), also known as Protor-1, is a 388 amino acid protein in Protor family, is thought to act as a tumor suppressor in breast and colorectal tumorigenesis. PRR5 is widely expressed and possesses two RICTOR interaction sites and a C-terminal Proline rich region. It promotes Rapamycin complex 2 (mTORC2) activity. There are four isoforms of PRR5 that are produced as a result of alternative splicing events and these isoforms play an important role in the modulation of platelet-derived growth factor signaling and in the regulation of PDGFR-beta expression.1) Johnstone CN, Castellvi-Bel S, Chang LM, et al. PRR5 encodes a conserved proline-rich protein predominant in kidney: analysis of genomic organization, expression, and mutation status in breast and colorectal carcinomas. Genomics 2005; 85:338-51. |
| Immunogen | PRR5 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human PRR5. The immunogen is located within amino acids 310 - 360 of PRR5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| PRR5 antibody can be used for detection of PRR5 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRR5 |
|---|---|
| Alternative Names | PRR5 Antibody: PP610 PROTOR1 PROTOR-1 FLJ20185k PP610 Proline-rich protein 5 Protein observed with Rictor-1 Protor-1 |
| Tissue Specificity | PRR5 antibody is predicted to not cross-react with other Protor protein family members. |
Application
Immunohistochemistry of PPR5 in mouse brain tissue with PPR5 antibody at 5 μg/mL.
Application
Immunofluorescence of PPR5 in mouse brain tissue with PPR5 antibody at 20 μg/mL.
Application
Western blot analysis of PRR5 in SK-N-SH cell lysate with PRR5 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | PRR5 antibody can be used for detection of PRR5 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PRR5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PRR5 Antibody is supplied in PBS containing 0.02% sodium azide. |
