PSIP1 encodes a multidomain adaptor protein that interacts with the nuclear import apparatus, lentiviral IN proteins and chromatin by means of an NLS, an IBD and additional chromatin-interacting domains.1) Llano, M., et al., (2004) J. Virol. 78 (17), 9524-9537.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PSIP1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
PSIP1 antibody can be used for detection of PSIP1 by ELISA at 1:12500. PSIP1 antibody can be used for detection of PSIP1 by western blot at 0.125 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Jurkat cells at 0.2-1 ug/ml.
Application
Antibody used in WB on mouse brain at 1:1000 (Lane 1: 5ug mouse brain cytoplasm Lane 2: 5ug mouse brain nucleus ).
Application
Antibody used in WB on Human Jurkat.
Positive Control
1) Cat. No. 1205 - Jurkat Cell Lysate
Application Notes
PSIP1 antibody can be used for detection of PSIP1 by ELISA at 1:12500. PSIP1 antibody can be used for detection of PSIP1 by western blot at 0.125 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store PSIP1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.