Application
Western blot analysis in A2058 cell line lysates (35ug/lane).| Product Name | PSMA1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Proteasome subunit alpha type-1, 30 kDa prosomal protein, PROS-30, Macropain subunit C2, Multicatalytic endopeptidase complex subunit C2, Proteasome component C2, Proteasome nu chain, PSMA1, HC2, NU, PROS30, PSC2 |
| Product description | The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Alternative splicing results in multiple transcript variants encoding distinct isoforms.1) Zhang, Y., et al. Biochem. Biophys. Res. Commun. 355(1):245-251(2007) |
| Immunogen | This PSMA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 234-262 amino acids from the C-terminal region of human PSMA1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PSMA1 |
|---|---|
| Alternative Names | Proteasome subunit alpha type-1 30 kDa prosomal protein PROS-30 Macropain subunit C2 Multicatalytic endopeptidase complex subunit C2 Proteasome component C2 Proteasome nu chain PSMA1 HC2 NU PROS30 PSC2 |
| Molecular Weight(MW) | 30 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis in A2058 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
