Application
Antibody used in WB on Human HeLa cells at 0.2-1 ug/ml.Application
Antibody used in WB on Human Jurkat 1 ug/ml.Application
Antibody used in WB on Human HepG2 at 1 ug/ml.Application
Antibody used in WB on Human 293T at 1 ug/ml.| Product Name | PSMB2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PSMB2, HC7-I, MGC104215, MGC126885 |
| Product description | The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMB2 is a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit.1) Gregory, S.G., (2006) Nature 441 (7091), 315-321 . |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PSMB2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| PSMB2 antibody can be used for detection of PSMB2 by ELISA at 1:312500. PSMB2 antibody can be used for detection of PSMB2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PSMB2 |
|---|---|
| Alternative Names | PSMB2 HC7-I MGC104215 MGC126885 |
| Molecular Weight(MW) | 23 kDa |
Application
Antibody used in WB on Human HeLa cells at 0.2-1 ug/ml.
Application
Antibody used in WB on Human Jurkat 1 ug/ml.
Application
Antibody used in WB on Human HepG2 at 1 ug/ml.
Application
Antibody used in WB on Human 293T at 1 ug/ml.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | PSMB2 antibody can be used for detection of PSMB2 by ELISA at 1:312500. PSMB2 antibody can be used for detection of PSMB2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store PSMB2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
