Application
Western blot analysis in mouse kidney tissue lysates (35ug/lane).Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with PSMD11 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of PSMD11 Antibody in 293, Jurkat cell line lysates (35ug/lane)| Product Name | PSMD11 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 26S proteasome non-ATPase regulatory subunit 11, 26S proteasome regulatory subunit RPN6, 26S proteasome regulatory subunit S9, 26S proteasome regulatory subunit p445, PSMD11 |
| Product description | The 26S proteasome (PSMD11) is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The protein is a non-ATPase subunit of the 19S regulator.1) Saito,A., Gene 203 (2), 241-250 (1997) |
| Immunogen | This PSMD11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 288-317 amino acids from the C-terminal region of human PSMD11. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | PSMD11 |
|---|---|
| Alternative Names | 26S proteasome non-ATPase regulatory subunit 11 26S proteasome regulatory subunit RPN6 26S proteasome regulatory subunit S9 26S proteasome regulatory subunit p445 PSMD11 |
| Molecular Weight(MW) | 47 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis in mouse kidney tissue lysates (35ug/lane).
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with PSMD11 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of PSMD11 Antibody in 293, Jurkat cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
