Application
Flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded mouse hepatocarcinoma reacted with PSME2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western Blot at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: PC-3 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | PSME2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Proteasome activator complex subunit 2, 11S regulator complex subunit beta, REG-beta, Activator of multicatalytic protease subunit 2, Proteasome activator 28 subunit beta, PA28b, PA28beta, PSME2 |
| Product description | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. PSME2 is the beta subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon.1) Conticello,S.G., Curr. Biol. 13 (22), 2009-2013 (2003) |
| Immunogen | This PSME2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61-91 amino acids from the Central region of human PSME2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PSME2 |
|---|---|
| Alternative Names | Proteasome activator complex subunit 2 11S regulator complex subunit beta REG-beta Activator of multicatalytic protease subunit 2 Proteasome activator 28 subunit beta PA28b PA28beta PSME2 |
| Molecular Weight(MW) | 27 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded mouse hepatocarcinoma reacted with PSME2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western Blot at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: PC-3 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
