Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with PTPN6 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of PTPN6 using rabbit polyclonal PTPN6 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PTPN6 gene (Lane 2).(2ug/ml)Application
Immunofluorescent analysis of U251 cells, using PTPN6 Antibody . Antibody was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).Application
Western blot analysis of PTPN6 Antibody in HL-60 cell line lysates (35ug/lane)| Product Name | PTPN6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Tyrosine-protein phosphatase non-receptor type 6, Hematopoietic cell protein-tyrosine phosphatase, Protein-tyrosine phosphatase 1C, PTP-1C, Protein-tyrosine phosphatase SHP-1, SH-PTP1, PTPN6, HCP, PTP1C |
| Product description | PTPN6 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling.1) Korporaal,S.J., Arterioscler. Thromb. Vasc. Biol. 29 (3), 372-379 (2009) |
| Immunogen | This PTPN6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 247-277 amino acids from the Central region of human PTPN6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For IF starting dilution is: 1:100 For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | PTPN6 |
|---|---|
| Alternative Names | Tyrosine-protein phosphatase non-receptor type 6 Hematopoietic cell protein-tyrosine phosphatase Protein-tyrosine phosphatase 1C PTP-1C Protein-tyrosine phosphatase SHP-1 SH-PTP1 PTPN6 HCP PTP1C |
| Molecular Weight(MW) | 68 kDa |
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with PTPN6 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of PTPN6 using rabbit polyclonal PTPN6 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PTPN6 gene (Lane 2).(2ug/ml)
Application
Immunofluorescent analysis of U251 cells, using PTPN6 Antibody . Antibody was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
Application
Western blot analysis of PTPN6 Antibody in HL-60 cell line lysates (35ug/lane)| Application Notes | For IF starting dilution is: 1:100 For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
