Application
ApplicationApplication
Antibody used in WB on Hum. Fetal Liver at 1 ug/ml.Application
Antibody used in WB on Hum. Fetal Lung at 1 ug/ml.Application
Antibody used in IHC on Human Intestine at 4.0-8.0 ug/ml.Application
Antibody used in WB on Human 293T at 0.5 ug/ml.Application
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.| Product Name | PUF60 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | PUF60 is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription.The protein encoded by this gene is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. There are two alternatively spliced transcript variants of this gene encoding different isoforms. There seems to be evidence of multiple polyadenylation sites for this gene.The protein encoded by this gene is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. There are two alternatively spliced transcript variants of this gene encoding different isoforms. There seems to be evidence of multiple polyadenylation sites for this gene.1) Gao, J., (2008) Genomics 91 (4), 347-355. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PUF60. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| PUF60 antibody can be used for detection of PUF60 by ELISA at 1:312500. PUF60 antibody can be used for detection of PUF60 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PUF60 |
|---|---|
| Alternative Names | PUF60 FIR FLJ31379 RoBPI SIAHBP1 VRJS |
| Molecular Weight(MW) | 58 kDa |
Application
Application
Application
Antibody used in WB on Hum. Fetal Liver at 1 ug/ml.
Application
Antibody used in WB on Hum. Fetal Lung at 1 ug/ml.
Application
Antibody used in IHC on Human Intestine at 4.0-8.0 ug/ml.
Application
Antibody used in WB on Human 293T at 0.5 ug/ml.
Application
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | PUF60 antibody can be used for detection of PUF60 by ELISA at 1:312500. PUF60 antibody can be used for detection of PUF60 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store PUF60 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
