Application
Immunohistochemistry of PUMA in human breast tissue with PUMA antibody at 2.5 μg/ml.Application
Immunohistochemistry of PUMA in human breast carcinoma with PUMA antibody at 10 μg/mL.Application
Immunofluorescence of PUMA in K562 cells with PUMA antibody at 10 μg/mL.Application
Immunofluorescence of PUMA in K562 cells with PUMA antibody at 20 μg/ml.Application
Western blot analysis of PUMA expression in K562 cell lysate with PUMA antibody at 2 μg /ml.| Product Name | PUMA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | PUMA Antibody: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.1) Nakano K, Vousden KH. PUMA, a novel proapoptotic gene, is induced by p53. Mol Cell. 2001;7(3):683-94. |
| Immunogen | PUMA antibody was raised against a synthetic peptide corresponding to 14 amino acids near the amino terminus of human PUMA-a. The sequence is identical between a and b forms of the PUMA proteins. The immunogen is located within the first 50 amino acids of |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| PUMA antibody can be used for detection of PUMA by Western blot at 2 μg/mL. Antibody can also detect PUMA by immunohistochemistry at 10 μg/mL. For immunofluorescence start at 10 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BBC3 |
|---|---|
| Alternative Names | PUMA Antibody: JFY1 PUMA JFY-1 Bcl-2-binding component 3 |
| Molecular Weight(MW) | 23 kDa |
| Sequence Similarities | 3) Cat. No. 17-004 - K-562 Cell Slide |
Application
Immunohistochemistry of PUMA in human breast tissue with PUMA antibody at 2.5 μg/ml.
Application
Immunohistochemistry of PUMA in human breast carcinoma with PUMA antibody at 10 μg/mL.
Application
Immunofluorescence of PUMA in K562 cells with PUMA antibody at 10 μg/mL.
Application
Immunofluorescence of PUMA in K562 cells with PUMA antibody at 20 μg/ml.
Application
Western blot analysis of PUMA expression in K562 cell lysate with PUMA antibody at 2 μg /ml.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | PUMA antibody can be used for detection of PUMA by Western blot at 2 μg/mL. Antibody can also detect PUMA by immunohistochemistry at 10 μg/mL. For immunofluorescence start at 10 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | PUMA antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PUMA Antibody is supplied in PBS containing 0.02% sodium azide. |
