Application
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
PUR4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis of PUR4 Antibody in HepG2 cell line lysates (35ug/lane)Application
Western Blot at 1:1000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | PUR4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | This PUR4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 184-213 amino acids from the N-terminal region of human PUR4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PFAS |
|---|---|
| Alternative Names | Phosphoribosylformylglycinamidine synthase FGAM synthase FGAMS Formylglycinamide ribonucleotide amidotransferase FGAR amidotransferase FGAR-AT Formylglycinamide ribotide amidotransferase PFAS KIAA0361 |
| Molecular Weight(MW) | 145 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
PUR4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis of PUR4 Antibody in HepG2 cell line lysates (35ug/lane)
Application
Western Blot at 1:1000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
