Application
Western blot analysis in A549,NCI-H292 cell line lysates (35ug/lane).Application
PURA Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | PURA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene product is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia.1) Inoue, T., et al. Prostate 69(8):861-873(2009) |
| Immunogen | This PURA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 259-287 amino acids from the C-terminal region of human PURA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For IHC-P starting dilution is: 1:50~100 For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PURA |
|---|---|
| Alternative Names | Transcriptional activator protein Pur-alpha Purine-rich single-stranded DNA-binding protein alpha PURA PUR1 |
| Molecular Weight(MW) | 35 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis in A549,NCI-H292 cell line lysates (35ug/lane).
Application
PURA Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For IHC-P starting dilution is: 1:50~100 For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
