Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PXN Antibody (Y118) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of PXN using rabbit polyclonal PXN Antibody (Y118) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with PXN gene (Lane 2).Application
Western Blot at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | PXN Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | PXN is a cytoskeletal adapter protein involved in organisation and function of focal adhesions, which are critical to cell adhesion and migration. This in turn plays a role in a wide variety of processes including embryogenesis, organogenesis, wound repair, inflammation and cancer. PXN contains LD motifs, LIM domains, SH3 and SH2 binding domains that serve as docking sites for cytoskeletal proteins, tyrosine kinases (e.g., FAK, Pyk 2, Src), serine/threonine kinases, GTPase activating proteins and other adaptor proteins (e.g., Actin, Vinculin, Crk). |
| Immunogen | This PXN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 94-125 amino acids from human PXN. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PXN |
|---|---|
| Alternative Names | Paxillin PXN |
| Molecular Weight(MW) | 65 kDa |
Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PXN Antibody (Y118) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of PXN using rabbit polyclonal PXN Antibody (Y118) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with PXN gene (Lane 2).
Application
Western Blot at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
