Application
Confocal immunofluorescent analysis of PYGO1 Antibody with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).Application
Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).Application
PYGO1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | PYGO1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | PYGO1 is involved in signal transduction through the Wnt pathway.1) Frank, B., et al. Carcinogenesis (2010) In press : |
| Immunogen | This PYGO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 382-409 amino acids from the C-terminal region of human PYGO1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PYGO1 |
|---|---|
| Alternative Names | Pygopus homolog 1 PYGO1 |
| Molecular Weight(MW) | 45 kDa |
Application
Confocal immunofluorescent analysis of PYGO1 Antibody with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Application
Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).
Application
PYGO1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
