tRNA-guanine transglycosylase (TGT; EC 2.4.2.29)
synthesizes queuosine (Q), which is found in tRNAs that recognize
NAU and NAC codons, encoding tyr, asn, asp, and his. Prokaryotic
TGT is a single protein of 43 kD. In contrast, mammalian TGT
appears to be a heterodimer consisting of a 60-kD subunit (USP14;
MIM 607274) and a 43-kD catalytic subunit (QTRT1) (Deshpande and
Katze, 2001 [PubMed 11255023]).1) Chen, Y.C., et al. RNA 16(5):958-968(2010)
Immunogen
This QTRT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 345-371 amino acids from the C-terminal region of human QTRT1.
Western blot analysis in SK-BR-3 cell line lysates (35ug/lane).
Application Notes
For WB starting dilution is: 1:1000
Additional Information
Form
Liquid
Storage Instructions
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.