Application
Western blot analysis of RAB21 in mouse kidney tissue lysate with RAB21 antibody at 1 μg/mL.Application
Immunofluorescence of RAB21 in human kidney tissue with RAB21 antibody at 20 μg/mL.| Product Name | RAB21 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RAB21 Antibody: KIAA0118, Ras-related protein Rab-21 |
| Product description | RAB21 Antibody: RAB21 is a member of a subfamily of small GTP-binding protein genes of the Ras superfamily that plays an important role in intracellular vesicular targeting. In non-polarized Caco-2 cells, RAB21 showed a general endoplasmic reticulum (ER)-like localization, while in polarized cells, RAB21 localized to apical vesicles. RAB21 has been shown to associate with integrin subunits and to be important for receptor entry into cells via RAB5/RAB21 endosomes. The GTPase-activating protein p120RasGAP regulates cell motility by controlling the return of the endocytosed integrins to the plasma membrane by competing with RAB21 for binding to overlapping sites on the α-tail of endocytosed integrin.1) Opdam FJ, Kamps G, Croes H, et al. Expression of Rab small GTPases in epithelial Caco-2 cells: Rab21 is an apically located GTP-binding protein in polarised intestinal epithelial cells. Eur. J. Cell Biol. 2000; 79:308-16. |
| Immunogen | RAB21 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human RAB21. The immunogen is located within the last 50 amino acids of RAB21. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| RAB21 antibody can be used for detection of RAB21 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RAB21 |
|---|---|
| Alternative Names | RAB21 Antibody: KIAA0118 Ras-related protein Rab-21 |
| Sequence Similarities | 2) Cat. No. 10-401 - Human Kidney Tissue Slide |
| Tissue Specificity | This antibody is predicted to not cross-react with other Ras-related proteins |
Application
Western blot analysis of RAB21 in mouse kidney tissue lysate with RAB21 antibody at 1 μg/mL.
Application
Immunofluorescence of RAB21 in human kidney tissue with RAB21 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1405 - Mouse Kidney Tissue Lysate |
|---|---|
| Application Notes | RAB21 antibody can be used for detection of RAB21 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | RAB21 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | RAB21 Antibody is supplied in PBS containing 0.02% sodium azide. |
