RAB3A is involved in exocytosis by regulating a late step in synaptic vesicle fusion. It could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal.1) Zahraoui, A., et al., (1989) J. Biol. Chem. 264:12394-12401..
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RAB3A.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
RAB3A antibody can be used for detection of RAB3A by ELISA at 1:15000. RAB3A antibody can be used for detection of RAB3A by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human Fetal Brain at 1.25 ug/ml.
Application Notes
RAB3A antibody can be used for detection of RAB3A by ELISA at 1:15000. RAB3A antibody can be used for detection of RAB3A by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store RAB3A antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt