Application
Antibody used in WB on Human Jurkat 2.5 ug/ml.Application
Antibody used in IHC on Human Lung at 4.0-8.0 ug/ml.| Product Name | RAD23A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RAD23A, HHR23A, MGC111083, HR23A |
| Product description | RAD23A is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in nucleotide excision repair (NER). This protein was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in nucleotide excision repair (NER). This protein was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.1) Ju, D. (2006) Biochem. Biophys. Res. Commun. 341 (2), 648-652. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RAD23A. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| RAD23A antibody can be used for detection of RAD23A by ELISA at 1:62500. RAD23A antibody can be used for detection of RAD23A by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RAD23A |
|---|---|
| Alternative Names | RAD23A HHR23A MGC111083 HR23A |
| Molecular Weight(MW) | 40 kDa |
Application
Antibody used in WB on Human Jurkat 2.5 ug/ml.
Application
Antibody used in IHC on Human Lung at 4.0-8.0 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | RAD23A antibody can be used for detection of RAD23A by ELISA at 1:62500. RAD23A antibody can be used for detection of RAD23A by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store RAD23A antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
