Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Product Name | RAD54B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | RAD54B belongs to the DEAD-like helicase superfamily. It shares similarity with Saccharomyces cerevisiae RAD54 and RDH54, both of which are involved in homologous recombination and repair of DNA. This protein binds to double-stranded DNA, and displays ATPase activity in the presence of DNA. This gene is highly expressed in testis and spleen, which suggests active roles in meiotic and mitotic recombination. Homozygous mutations of this gene were observed in primary lymphoma and colon cancer.The protein encoded by this gene belongs to the DEAD-like helicase superfamily. It shares similarity with Saccharomyces cerevisiae RAD54 and RDH54, both of which are involved in homologous recombination and repair of DNA. This protein binds to double-stranded DNA, and displays ATPase activity in the presence of DNA. This gene is highly expressed in testis and spleen, which suggests active roles in meiotic and mitotic recombination. Homozygous mutations of this gene were observed in primary lymphoma and colon cancer.1) Brys, M., (2007) Pol J Pathol 58 (1), 3-6. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RAD54B. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| RAD54B antibody can be used for detection of RAD54B by ELISA at 1:62500. RAD54B antibody can be used for detection of RAD54B by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RAD54B |
|---|---|
| Alternative Names | RAD54B FSBP RDH54 |
| Molecular Weight(MW) | 103 kDa |
Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | RAD54B antibody can be used for detection of RAD54B by ELISA at 1:62500. RAD54B antibody can be used for detection of RAD54B by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store RAD54B antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
