Application
Antibody used in IHC on Human Kidney at 4.0-8.0 ug/ml.Application
Antibody used in WB on Human Jurkat cells at 0.2-1 ug/ml.| Product Name | RAE1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | RAE1 is a homolog of yeast Rae1. It contains four WD40 motifs, and has been shown to localize to distinct foci in the nucleoplasm, to the nuclear rim, and to meshwork-like structures throughout the cytoplasm. This gene is thought to be involved in nucleocytoplasmic transport, and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton.Mutations in the Schizosaccharomyces pombe Rae1 and Saccharomyces cerevisiae Gle2 genes have been shown to result in accumulation of poly (A)-containing mRNA in the nucleus, suggesting that the encoded proteins are involved in RNA export. The protein encoded by this gene is a homolog of yeast Rae1. It contains four WD40 motifs, and has been shown to localize to distinct foci in the nucleoplasm, to the nuclear rim, and to meshwork-like structures throughout the cytoplasm. This gene is thought to be involved in nucleocytoplasmic transport, and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton. Alternatively spliced transcript variants encoding the same protein have been found for this gene.1) Blower, M.D., (2005) Cell 121 (2), 223-234. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RAE1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| RAE1 antibody can be used for detection of RAE1 by ELISA at 1:312500. RAE1 antibody can be used for detection of RAE1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | DogHumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RAE1 |
|---|---|
| Alternative Names | RAE1 MIG14 MRNP41 Mnrp41 dJ481F12.3 dJ800J21.1 |
| Molecular Weight(MW) | 40 kDa, 41 kDa |
| Entrez Gene | 8480 |
|---|---|
| Protein Accession | NP_001015885 |
Application
Antibody used in IHC on Human Kidney at 4.0-8.0 ug/ml.
Application
Antibody used in WB on Human Jurkat cells at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | RAE1 antibody can be used for detection of RAE1 by ELISA at 1:312500. RAE1 antibody can be used for detection of RAE1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store RAE1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
