| Product Name | RANKL Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | RANKL is an osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. Important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. Plays an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. |
| Immunogen | Recombinant human RANKL. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAFACSWB |
| ELISA: (direct and indirect: 1:2,000-1:10,000). Flow Cytometry: (1:500-1:1,000). Western Blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 with 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | TNFSF11 |
|---|---|
| Alternative Names | TRANCE ODF OPGL TNFSF11 CD254 |
| Tissue Specificity | Recognizes human RANKL. Detects a band of ~35kDa band by Western blot. |
| Application Notes | ELISA: (direct and indirect: 1:2,000-1:10,000). Flow Cytometry: (1:500-1:1,000). Western Blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 with 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Stable for at least 6 months after receipt when stored at -20˚C. |
| Storage Buffer | Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives. |
