Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rb (Ab-807).Application
Western blot analysis of lysed extracts from K562 cells using Rb (Ab-807).| Product Name | Rb Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rb operates in the midst of the cell cycle clock apparatus. Its main role is to act as a signal transducer connecting the cell cycle clock with the transcriptional machinery (Ref.1). It plays an important role in the Rb/ E2F pathway in cell proliferation, cell fate determination, and cancer (Ref.2). Cellular senescence is a stable form of cell cycle arrest that limits proliferation of damaged cells and act as a natural barrier to cancer progression. A distinct heterochromatic structure that accumulates in senescent human fibroblasts, designated as SAHF (Senescence-Associated Heterochromatic Foci). SAHF formation coincides with recruitment of heterochromatin proteins and the Rb protein to E2F-responsive promoters and is associated with the stable repression of E2F target genes. Both SAHF formation and the silencing of E2F target genes depended on the integrity of the Rb pathway and do not occur in reversibly arrested cells (Ref.3). Rb activates transcription of the c-Jun gene through the SP1-binding site within the c-Jun promoter1) Roesch A, et al. (2005) Mod Pathol. 18 (4): 565-572. Chadee DN, et al. (2004) Nat Cell Biol. 6 (8): 770-776. Knudsen ES, et al. (1997) Mol Cell Biol. 17 (10): 5771-5783. Knudsen ES, et al. (1996) J Biol Chem. 271 (14): 8313-8320. |
| Immunogen | Rb (Ab-807) antibody was raised against a peptide sequence around aa.805~809 (Y-I-S-P-L) derived from Human Rb. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RB1 |
|---|---|
| Alternative Names | RB pRb OSRC pp110 p105-Rb CDC2 CDC28A CDKN1 P34CDC2 P105-RB PP105 PP110 RB-1 RB1 |
| Molecular Weight(MW) | 110 kDa |
| Tissue Specificity | This antibody detects endogenous level of total Rb protein. |
| Entrez Gene | 5925 |
|---|---|
| Protein Accession | NP_000312.2 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rb (Ab-807).
Application
Western blot analysis of lysed extracts from K562 cells using Rb (Ab-807).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
