Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rb (Phospho-Ser795). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using Rb (Phospho-Ser795).Application
Western blot analysis of lysed extracts from K562 cells untreated (Lane 1) or treated with serum (lane 2) using Rb (Phospho-Ser795).| Product Name | Rb (phospho Ser795) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RB, pRb, OSRC, pp110, p105-Rb, ASK1, MAPKKK5, MEKK5, P105-RB, PP105, PP110, RB-1, RB1 |
| Product description | Key regulator of entry into cell division that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.1) Fang Sun, Hanjiang Fu, Qin Liu, et al. (2008) Downregulation of CCND1 and CDK6 by miR-34a induces cell cyclearrest. FEBS Letters, 582:1564–1568. |
| Immunogen | Rb (Phospho-Ser795) antibody was raised against a peptide sequence around phosphorylation site of serine 795 (P-S-S (p) -P-L) derived from Human Rb. |
| Modification | p-Ser795 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RB1 |
|---|---|
| Alternative Names | RB pRb OSRC pp110 p105-Rb ASK1 MAPKKK5 MEKK5 P105-RB PP105 PP110 RB-1 RB1 |
| Molecular Weight(MW) | 110 kDa |
| Tissue Specificity | This antibody detects endogenous level of Rb only when phosphorylated at serine 795. |
| Entrez Gene | 5925 |
|---|---|
| Protein Accession | NP_000312.2 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rb (Phospho-Ser795). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using Rb (Phospho-Ser795).
Application
Western blot analysis of lysed extracts from K562 cells untreated (Lane 1) or treated with serum (lane 2) using Rb (Phospho-Ser795).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
