Application
Flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of RB1 Antibody in 293 cell line lysates (35ug/lane)(2ug/ml)Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with RB1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western Blot at 1:8000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | RB1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Retinoblastoma-associated protein, p105-Rb, pRb, Rb, pp110, RB1 |
| Product description | RB1 is a negative regulator of the cell cycle and was the first tumor suppressor gene found. This protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1.1) Connell-Crowley,L., et.al., Mol. Biol. Cell(2), 287-301 (1997) |
| Immunogen | This RB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 858-886 amino acids from the C-terminal region of human RB1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | RB1 |
|---|---|
| Alternative Names | Retinoblastoma-associated protein p105-Rb pRb Rb pp110 RB1 |
| Molecular Weight(MW) | 106 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of RB1 Antibody in 293 cell line lysates (35ug/lane)(2ug/ml)
Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with RB1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western Blot at 1:8000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
