RBAK is a nuclear protein which interacts with the tumor suppressor retinoblastoma 1. The two interacting proteins are thought to act as a transcriptional repressor for promoters which are activated by the E2F1 transcription factor. This protein contains a Kruppel-associated box (KRAB), which is a transcriptional repressor motif.1) Hofman, K., et al., (2003) J. Mol. Endocrinol. 31 (3), 583-596.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RBAK.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
RBAK antibody can be used for detection of RBAK by ELISA at 1:1562500. RBAK antibody can be used for detection of RBAK by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human NTERA2 at 1.25 ug/ml.
Positive Control
1) NTERA2 Cell Lysate
Application Notes
RBAK antibody can be used for detection of RBAK by ELISA at 1:1562500. RBAK antibody can be used for detection of RBAK by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store RBAK antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt