Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.Application
Western Blot at 1:2000 dilution Lane 1: HT-29 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: Raji whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | RBL2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Retinoblastoma-like protein 2, 130 kDa retinoblastoma-associated protein, p130, Retinoblastoma-related protein 2, RBR-2, pRb2, RBL2, RB2 |
| Product description | RBL2 is a key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. RBL2 may act as a tumor suppressor.1) Schaffer, B.E., et al. Cancer Res. 70(10):3877-3883(2010) |
| Immunogen | This RBL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 165-194 amino acids from the N-terminal region of human RBL2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:2000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RBL2 |
|---|---|
| Alternative Names | Retinoblastoma-like protein 2 130 kDa retinoblastoma-associated protein p130 Retinoblastoma-related protein 2 RBR-2 pRb2 RBL2 RB2 |
| Molecular Weight(MW) | 128 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Application
Western Blot at 1:2000 dilution Lane 1: HT-29 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: Raji whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
