Application
Immunohistochemistry of RBM35B in human lung tissue with RBM35B antibody at 5 μg/mL.Application
Immunofluorescence of RBM35B in human lung tissue cells with RBM35B antibody at 20 μg/mL.Application
Western blot analysis of RBM35B in human lung tissue lysate with RBM35B antibody at 1 μg/mL.| Product Name | RBM35B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RBM35B Antibody: RBM35B, RBM35B, PP7059, Epithelial splicing regulatory protein 2, RNA-binding motif protein 35B |
| Product description | RBM35B Antibody: RBM35A, also known as ESRP2, is a mRNA splicing factor that with its related protein RBM35A (ESRP1) are coordinators of an epithelial cell-type-specific splicing program. Both RBM35B and RBM35A are involved in posttranscriptional regulation of a number of genes such as FGFR2, CD44, CTNND1, and ENAH by exerting a differential effect on protein translation via 5' UTRs of mRNAs, suggesting that these proteins are global regulators of an epithelial regulatory network. Loss of this global ESRP-regulated epithelial splicing programme induces the phenotypic changes in cell morphology that are observed during the epithelial-mesenchymal transition.1) Warzecha CC, Sato TK, Nabet B, et al. ESRP1 and ESRP2 are epithelial cell-type-specific regulators of FGFR2 splicing. Mol Cell. 2009; 33:591-601. |
| Immunogen | RBM35B antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human RBM35B. The immunogen is located within the last 50 amino acids of RBM35B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| RBM35B antibody can be used for detection of RBM35B by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ESRP2 |
|---|---|
| Alternative Names | RBM35B Antibody: RBM35B RBM35B PP7059 Epithelial splicing regulatory protein 2 RNA-binding motif protein 35B |
| Sequence Similarities | 2) Cat. No. 10-101 - Human Lung Tissue Slide |
| Tissue Specificity | RBM35B antibody is predicted to not cross-react with other RBM35/ESRP family members. At least three isoforms of RBM35B are known to exist; this antibody will detect all three. |
Application
Immunohistochemistry of RBM35B in human lung tissue with RBM35B antibody at 5 μg/mL.
Application
Immunofluorescence of RBM35B in human lung tissue cells with RBM35B antibody at 20 μg/mL.
Application
Western blot analysis of RBM35B in human lung tissue lysate with RBM35B antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1302 - Human Lung Tissue Lysate |
|---|---|
| Application Notes | RBM35B antibody can be used for detection of RBM35B by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | RBM35B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | RBM35B Antibody is supplied in PBS containing 0.02% sodium azide. |
