RBM4 contains 1 CCHC-type zinc finger and 2 RRM (RNA recognition motif) domains. It may play a role in alternative splice site selection during pre-mRNA processing. RBM4 is down-regulated in fetal Down syndrome (DS) brain.1) Lin, J.C., (2007) Proc. Natl. Acad. Sci. U.S.A. 104 (7), 2235-2240.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RBM4.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
WB
RBM4 antibody can be used for detection of RBM4 by ELISA at 1:1562500. RBM4 antibody can be used for detection of RBM4 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human MCF7 cells at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1219 - MCF7 Cell Lysate
Application Notes
RBM4 antibody can be used for detection of RBM4 by ELISA at 1:1562500. RBM4 antibody can be used for detection of RBM4 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store RBM4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.