Application
Antibody used in WB on Human HepG2.Application
Antibody used in WB on Human HepG2 cells at 0.2-1 ug/ml.| Product Name | RBM5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RBM5, FLJ39876, G15, H37, LUCA15, RMB5 |
| Product description | RBM5 is a component of the spliceosome A complex.It regulates alternative splicing of a number of mRNAs. RBM5 may modulate splice site pairing after recruitment of the U1 and U2 snRNPs to the 5' and 3' splice sites of the intron. RBM5 may both positively and negatively regulate aopotosis by regulating the alternative splicing of several genes involved in this process, including FAS and CASP2/caspase-2. In the case of FAS, promotes exclusion of exon 6 thereby producing a soluble form of FAS that inhibits apoptosis. In the case of CASP2/caspase-2, RBM5 promotes exclusion of exon 9 thereby producing a catalytically active form of CASP2/Caspase-2 that induces apoptosis.1) Oh, J.J., (2008) Hum. Genet. 123 (1), 55-64. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RBM5. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| RBM5 antibody can be used for detection of RBM5 by ELISA at 1:312500. RBM5 antibody can be used for detection of RBM5 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RBM5 |
|---|---|
| Alternative Names | RBM5 FLJ39876 G15 H37 LUCA15 RMB5 |
| Molecular Weight(MW) | 92 kDa |
Application
Antibody used in WB on Human HepG2.
Application
Antibody used in WB on Human HepG2 cells at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | RBM5 antibody can be used for detection of RBM5 by ELISA at 1:312500. RBM5 antibody can be used for detection of RBM5 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store RBM5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
