Application
Western Blot at 1:1000 dilution Lane 1: 293 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis of anti-PHB2 Antibody in 293 cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PHB2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | REA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Prohibitin-2, B-cell receptor-associated protein BAP37, D-prohibitin, Repressor of estrogen receptor activity, PHB2 {ECO:0000312|EMBL:AAH147661, ECO:0000312|HGNC:HGNC:30306} |
| Product description | Acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. Functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. Competes with NCOA1 for modulation of ER transcriptional activity. Probably involved in regulating mitochondrial respiration activity and in aging.1) Takata,H., Curr. Biol. 17 (15), 1356-1361 (2007) |
| Immunogen | This REA (PHB2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 197-226 amino acids from the C-terminal region of human REA (PHB2). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PHB2 |
|---|---|
| Alternative Names | Prohibitin-2 B-cell receptor-associated protein BAP37 D-prohibitin Repressor of estrogen receptor activity PHB2 {ECO:0000312|EMBL:AAH147661 ECO:0000312|HGNC:HGNC:30306} |
| Molecular Weight(MW) | 33 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western Blot at 1:1000 dilution Lane 1: 293 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis of anti-PHB2 Antibody in 293 cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PHB2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
