Application
This antibody stained 4% PFA, paraffin-embedded sections of murine cecum tissue (with positive cells infiltrating the Lamina Propia of a Trichuris muris infected mouse). The primary antibody was incubated at 4 ng/mL overnight at 4˚C and the secondary antibody was a cyanine-3 conjugated Donkey anti-Rabbit (Jackson ImmunoResearch). Heat induced antigen retrieval with a 100mM Citric Acid was useApplication
To detect Murine RELM-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine RELM-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect Murine RELM-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine RELM-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect Murine RELM-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Murine RELM-alpha (38-148) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine RELM-alpha.| Product Name | RELMa Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HIMF, Xcp2, Fizz1, RELMa, Fizz-1, RELMalpha, RELM-alpha, 1810019L16Rik, Himf, Pmng1, Resistin-like alpha, Cysteine-rich secreted protein A12-gamma, |
| Product description | A family of resistin-like molecules (RELMs) has been identified in rodents and humans. RELM alpha belongs to a unique family of tissue-specific cytokines termed FIZZ (found in inflammatory zone) and RELM. The three known members of this family; Resistin, RELM alpha and RELM beta are 85-94 amino acid secreted proteins sharing a conserved C-terminal domain. RELM alpha and Resistin are secreted exclusively by adipocytes while RELM beta is expressed in the epithelium of the colon and small bowel. The RELMs together with resistin comprise a class of tissue-specific signaling molecules. The physiological role and molecular targets of RELM alpha are still unknown. |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mRELMα. Murine RELMα specific antibody was purified by affinity chromatography employing immobilized mRELMα matrix. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA: Indirect: To detect mRELMa by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mRELMa. Sandwich To detect mRELMa by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine RELMa as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mRELMa. Western Blot: To detect mRELMa by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mRELMa is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Gene Symbol | Retnla |
|---|---|
| Alternative Names | HIMF Xcp2 Fizz1 RELMa Fizz-1 RELMalpha RELM-alpha 1810019L16Rik Himf Pmng1 Resistin-like alpha Cysteine-rich secreted protein A12-gamma |
Application
This antibody stained 4% PFA, paraffin-embedded sections of murine cecum tissue (with positive cells infiltrating the Lamina Propia of a Trichuris muris infected mouse). The primary antibody was incubated at 4 ng/mL overnight at 4˚C and the secondary antibody was a cyanine-3 conjugated Donkey anti-Rabbit (Jackson ImmunoResearch). Heat induced antigen retrieval with a 100mM Citric Acid was use
Application
To detect Murine RELM-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine RELM-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect Murine RELM-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine RELM-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect Murine RELM-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Murine RELM-alpha (38-148) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine RELM-alpha.| Application Notes | ELISA: Indirect: To detect mRELMa by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mRELMa. Sandwich To detect mRELMa by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine RELMa as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mRELMa. Western Blot: To detect mRELMa by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mRELMa is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | RELMa antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
