Application
This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 1.0 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval. Optimal concentrations and conditions may vary.Application
To detect Human RELM-beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human RELM-beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect Human RELM-beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human RELM-beta (38-109) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human RELM-beta.| Product Name | RELMb Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | XCP2, FIZZ1, FIZZ2, HXCP2, RELMb, RELMbeta, RELM-beta, CCRG, RETNL2, UNQ408/PRO770, Resistin-like beta, Colon and small intestine-specific cysteine-rich protein, |
| Product description | RELM beta, is a secreted protein, a probable hormone, expressed only in the gastrointestinal tract, particularly the colon. The biological functions of RELM beta and its molecular targets, are not fully known but, it has been suggested that it plays a regulatory role during inflammation and may also act to establish links among adipose tissue, the intestine and the liver. Interestingly the molecular structure of RELM beta is highly homologous to that of the adipose-derived cytokine Resistin and RELM alpha. |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mRELMb. Human RELMb specific antibody was purified by affinity chromatography employing immobilized RELMb matrix. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| ELISA: Indirect: To detect hRELMb by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hRELMb. Sandwich: To detect hRELMb by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. Western Blot: To detect hRELMb by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hRELMb is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RETNLB |
|---|---|
| Alternative Names | XCP2 FIZZ1 FIZZ2 HXCP2 RELMb RELMbeta RELM-beta CCRG RETNL2 UNQ408/PRO770 Resistin-like beta Colon and small intestine-specific cysteine-rich protein |
Application
This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 1.0 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval. Optimal concentrations and conditions may vary.
Application
To detect Human RELM-beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human RELM-beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect Human RELM-beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human RELM-beta (38-109) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human RELM-beta.| Application Notes | ELISA: Indirect: To detect hRELMb by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hRELMb. Sandwich: To detect hRELMb by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. Western Blot: To detect hRELMb by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hRELMb is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | RELMb antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
