Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using Retinoblastoma (Phospho-Ser608) antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from JK cells (Lane 2), using Retinoblastoma (Phospho-Ser608) Antibody. The lane on the left is treated with antigen-specific peptide.| Product Name | Retinoblastoma (phospho Ser608) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | P105-RB, PP105, PP110, RB1 |
| Product description | Retinoblastoma (RB) is an embryonic malignant neoplasm of retinal origin. It almost always presents in early childhood and is often bilateral. Spontaneous regression ('cure') occurs in some cases.1) Lee W.-H., Nature 329:642-645(1987). |
| Immunogen | Retinoblastoma (phospho Ser608) antibody was raised against a peptide sequence around phosphorylation site of Serine 608 (Y-L-S (p)-P-V) derived from Human Retinoblastoma. |
| Modification | p-Ser608 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RB1 |
|---|---|
| Alternative Names | P105-RB PP105 PP110 RB1 |
| Molecular Weight(MW) | 110 kDa |
| Tissue Specificity | The antibody detects endogenous levels of Retinoblastoma only when phosphorylated at serine 608. |
| Entrez Gene | 5925 |
|---|---|
| Protein Accession | NP_000312.2 |
Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using Retinoblastoma (Phospho-Ser608) antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from JK cells (Lane 2), using Retinoblastoma (Phospho-Ser608) Antibody. The lane on the left is treated with antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
