Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with Phospho-RGS19-pS151.ctrl antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of anti-RGS19 Antibody (S151) in mouse muscle tissue lysates (35ug/lane).| Product Name | RGS19 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Regulator of G-protein signaling 19, RGS19, G-alpha-interacting protein, GAIP, RGS19, GAIP, GNAI3IP |
| Product description | RGS19 enhances the intrinsic GTPase-activating protein activity of the Galphai3 protein, which stimulates autophagy by favoring the GDP-bound form of Galphai3. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole).1) Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005) |
| Immunogen | This RGS19 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 131-156 amino acids from human RGS19. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RGS19 |
|---|---|
| Alternative Names | Regulator of G-protein signaling 19 RGS19 G-alpha-interacting protein GAIP RGS19 GAIP GNAI3IP |
| Molecular Weight(MW) | 25 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with Phospho-RGS19-pS151.ctrl antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of anti-RGS19 Antibody (S151) in mouse muscle tissue lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
