Application
Western blot analysis of RGS4 Antibody in MDA-MB231 cell line lysates (35ug/lane)Application
Western blot analysis of RGS4 Antibody in mouse brain tissue lysates (35ug/lane)Application
Western blot analysis of RGS4 using rabbit polyclonal RGS4 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RGS4 gene.| Product Name | RGS4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 4 belongs to this family. All RGS proteins share a conserved 120-amino acid sequence termed the RGS domain. Regulator of G protein signaling 4 protein is 37% identical to RGS1 and 97% identical to rat Rgs4. This protein negatively regulate signaling upstream or at the level of the heterotrimeric G protein and is localized in the cytoplasm.1) Heximer,S.P., et.al., Proc. Natl. Acad. Sci. U.S.A. 94 (26), 14389-14393 (1997) |
| Immunogen | This RGS4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-174 amino acids from the Central region of human RGS4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human,Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RGS4 |
|---|---|
| Alternative Names | Regulator of G-protein signaling 4 RGP4 RGS4 RGS4 |
| Molecular Weight(MW) | 23 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Western blot analysis of RGS4 Antibody in MDA-MB231 cell line lysates (35ug/lane)
Application
Western blot analysis of RGS4 Antibody in mouse brain tissue lysates (35ug/lane)
Application
Western blot analysis of RGS4 using rabbit polyclonal RGS4 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RGS4 gene.| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
