Application
Immunohistochemistry of RHAMM in human stomach tissue with RHAMM antibody at 2.5 μg/mL.Application
Western blot analysis of RHAMM in rat stomach tissue lysate with RHAMM antibody at 0.5 μg/mL.Application
Immunofluorescence of RHAMM in human stomach tissue with RHAM antibody at 20 μg/mL.| Product Name | RHAMM Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RHAMM Antibody: CD168, IHABP, RHAMM, Hyaluronan mediated motility receptor, Intracellular hyaluronic acid-binding protein |
| Product description | RHAMM Antibody: The hyaluronan-mediated motility receptor, also known as RHAMM, was initially identified as a soluble protein that could be released by sub-confluent migrating cells, promoting cell motility and invasion via interactions with hyaluronan (HA) and the cell surface. While RHAMM is normally poorly expressed in most normal tissues and is not required for embryonic development or normal cell homeostasis functions, its expression is increased during wound repair in response to hypoxia and fibrogenic factors. However, its overexpression is transforming in multiple types of cancers and is required for maintaining RAS transformation. RHAMM associates with BRCA1 and BARD1, attenuating the mitotic-spindle-promoting activity of RHAMM, which may contribute to tumor progression by promoting genomic instability.1) Hardwick C, Hoare K, Owens R, et al. Molecular cloning of a novel hyaluronan receptor that promotes tumor cell motility. J. Cell Biol. 1992; 117:1343-50. |
| Immunogen | RHAMM antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human RHAMM. The immunogen is located within amino acids 80 - 130 of RHAMM. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| RHAMM antibody can be used for detection of RHAMM by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HMMR |
|---|---|
| Alternative Names | RHAMM Antibody: CD168 IHABP RHAMM Hyaluronan mediated motility receptor Intracellular hyaluronic acid-binding protein |
| Sequence Similarities | 2) Cat. No. 10-801 - Human Stomach Tissue Slide |
Application
Immunohistochemistry of RHAMM in human stomach tissue with RHAMM antibody at 2.5 μg/mL.
Application
Western blot analysis of RHAMM in rat stomach tissue lysate with RHAMM antibody at 0.5 μg/mL.
Application
Immunofluorescence of RHAMM in human stomach tissue with RHAM antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1475 - Rat Stomach Tissue Lysate |
|---|---|
| Application Notes | RHAMM antibody can be used for detection of RHAMM by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | RHAMM antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | RHAMM Antibody is supplied in PBS containing 0.02% sodium azide. |
