Application
Western blot analysis of extract from HeLa cells using Ring1A Antibody.| Product Name | Ring1A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RNF1, RING1A, RNF1, RING1 |
| Product description | Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity.1) Cao R., Tsukada Y., Zhang Y. Mol. Cell 20:845-854 (2005) Satijn D. P. E., Gunster M. J., van der Vlag J., Hamer K. M., Schul W., Alkema M. J., Saurin A. J., Freemont P. S., van Driel R., Otte A. P. Mol. Cell. Biol. 17:4105-4113 (1997) |
| Immunogen | Ring1A antibody was raised against a peptide sequence around aa.35~39 ( I-A-V-S-P) derived from Human Ring1A. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RING1 |
|---|---|
| Alternative Names | RNF1 RING1A RNF1 RING1 |
| Molecular Weight(MW) | 58 kDa |
| Tissue Specificity | This antibody detects endogenous level of total Ring1A protein. |
| Entrez Gene | 6015 |
|---|---|
| Protein Accession | NP_002922.2 |
Application
Western blot analysis of extract from HeLa cells using Ring1A Antibody.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
