Application
Immunocytochemistry of RNAse H2A in HeLa cells with RNAse H2A antibody at 2 μg/mL.Application
Western blot analysis of RNAse H2A in HeLa cell lysate with RNAse H2A antibody at 1 μg/mL.Application
Immunofluorescence of RNase H2A in Hela cells with RNAse H2A antibody at 5 μg/mL.| Product Name | RNAse H2A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RNAse H2A Antibody: AGS4, JUNB, RNHL, RNHIA, RNASEHI, Ribonuclease H2 subunit A, Aicardi-Goutieres syndrome 4 protein, RNase H2 subunit A |
| Product description | RNAse H2A Antibody: Ribonucleases (RNAses) H are enzymes that hydrolyze the RNA strands of RNA/DNA hybrids. The major role of these enzymes is to remove the RNA strand from the RNA/DNA hybrids that form during DNA replication and repair. RNAse H2 is made up of three subunits; all three are required for RNAse activity. Recent evidence has demonstrated that mutations in RNAse H2A or any of the other subunits result in Aicardi-Goutieres syndrome (AGS), a neurological disorder with similar symptoms to viral brain infections including high levels of IFN-alpha in the cerebral spinal fluid. Similar conditions are observed with mutations in TREX1, a single-stranded DNA exonuclease, suggesting that RNAse H2 and TREX1 may have similar roles, and that mutations in any of these genes lead to an accumulation of intracellular nucleic acids, triggering an inflammatory response through activation of the innate immune system.1) Stein H and Hausen P. Enzyme from calf thymus degrading the RNA moiety of DNA-RNA hybrids: effect on DNA-dependent RNA polymerase. Science 1969; 166:393-5. |
| Immunogen | RNAse H2A antibody was raised against a 17 amino acid synthetic peptide near the center of human RNAse H2A. The immunogen is located within amino acids 190 - 240 of RNAse H2A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| RNAse H2A antibody can be used for detection of RNAse H2A by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 4 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RNASEH2A |
|---|---|
| Alternative Names | RNAse H2A Antibody: AGS4 JUNB RNHL RNHIA RNASEHI Ribonuclease H2 subunit A Aicardi-Goutieres syndrome 4 protein RNase H2 subunit A |
| Molecular Weight(MW) | Predicted: 33 kDa Observed: 33 kDa |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
Application
Immunocytochemistry of RNAse H2A in HeLa cells with RNAse H2A antibody at 2 μg/mL.
Application
Western blot analysis of RNAse H2A in HeLa cell lysate with RNAse H2A antibody at 1 μg/mL.
Application
Immunofluorescence of RNase H2A in Hela cells with RNAse H2A antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | RNAse H2A antibody can be used for detection of RNAse H2A by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 4 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | RNAse H2A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | RNAse H2A Antibody is supplied in PBS containing 0.02% sodium azide. |
