Application
Immunocytochemistry of RNF121 in MCF7 cells with RNF121 antibody at 10 μg/ml.Application
Western blot analysis of RNF121 in MCF7 cell lysate with RNF121 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Product Name | RNF121 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ring finger protein 121 |
| Product description | RNF121 is an evolutionarily conserved E3 ligase ring finger protein that is expressed in the endoplasmic reticulum (1). It acts downstream of the unfolded protein response regulator protein kinase-like endoplasmic reticulum kinase (PERK). Overexpression of RNF121 during the early stages of gonad development led to aberrations in germline development and gonad migration, suggesting that it plays a role in endoplasmic reticulum-associated degradation machinery (1).1) Darom A, Bening-Abu-Shach U, and Broday L. RNF-121 is an endoplasmic reticulum-membrane E3 ubiquitin ligase involved in the regulation of B-integrin. Mol. Biol. Cell 2010; 21:1788-98. |
| Immunogen | RNF121 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human RNF121. The immunogen is located within amino acids 260 - 310 of RNF121. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| RNF121 antibody can be used for detection of RNF121 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunocytochemistry at 10 μg/ml.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RNF121 |
|---|---|
| Alternative Names | Ring finger protein 121 |
| Molecular Weight(MW) | Predicted: 24, 27, 36 kDa Observed: 28 kDa |
| Sequence Similarities | 2) Cat. No. 17-012 - MCF7 Cell Slide |
| Tissue Specificity | RNF121 antibody is human, mouse and rat reactive. Multiple isoforms of RNF121 are known to exist. |
Application
Immunocytochemistry of RNF121 in MCF7 cells with RNF121 antibody at 10 μg/ml.
Application
Western blot analysis of RNF121 in MCF7 cell lysate with RNF121 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1219 - MCF7 Cell Lysate |
|---|---|
| Application Notes | RNF121 antibody can be used for detection of RNF121 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunocytochemistry at 10 μg/ml.: |
| Form | Liquid |
|---|---|
| Storage Instructions | RNF121 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | RNF121 antibody is supplied in PBS containing 0.02% sodium azide. |
