Application
Western Blot at 1:2000 dilution + CEM whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | RNF126 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | E3 ubiquitin-protein ligase RNF126, 632-, RING finger protein 126 {ECO:0000312|HGNC:HGNC:21151}, RNF126 ( HGNC:21151 ) |
| Product description | E3 ubiquitin-protein ligase that regulates several biological processes through ubiquitination of various target proteins. Depending on the associated E2 ligase, mediates 'Lys- 48'- and 'Lys-63'-linked polyubiquitination of substrates. Through their polyubiquitination, may play a role in the endosomal sorting and degradation of several membrane receptors including EGFR, FLT3, MET and CXCR4. May also be part of a BAG6-dependent quality control process ensuring that proteins of the secretory pathway that are mislocalized to the cytosol are degraded by the proteasome. May provide the ubiquitin ligase activity associated with the BAG6 complex and be responsible for ubiquitination of the mislocalized proteins and their targeting to the proteasome (PubMed: 24981174 ). May also play a role in the endosomal recycling of IGF2R, the cation-independent mannose-6-phosphate receptor (PubMed: 24275455 ). By ubiquitinating CDKN1A/p21 and targeting it for degradation, may also promote cell proliferation (PubMed: 23026136 ). May monoubiquitinate AICDA (PubMed: 23277564 ).1) Ota T.,et al.Nat. Genet. 36:40-45(2004). |
| Immunogen | This RNF126 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 84-115 amino acids from the Central region of human RNF126. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:2000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RNF126 |
|---|---|
| Alternative Names | E3 ubiquitin-protein ligase RNF126 632- RING finger protein 126 {ECO:0000312|HGNC:HGNC:21151} RNF126 ( HGNC:21151 ) |
| Molecular Weight(MW) | 36 kDa |
Application
Western Blot at 1:2000 dilution + CEM whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
