The protein encoded by RNF135 contains a RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions. This gene is located in a chromosomal region known to be frequently deleted in patients with neurofibromatosis.1) Petroziello, J., (2004) Oncogene 23(46), 7734-45.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RNF135.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
RNF135 antibody can be used for detection of RNF135 by ELISA at 1:62500. RNF135 antibody can be used for detection of RNF135 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
RNF135 antibody can be used for detection of RNF135 by ELISA at 1:62500. RNF135 antibody can be used for detection of RNF135 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store RNF135 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.