Application
Antibody used in IHC on Human kidney.Application
Antibody used in WB on Human Jurkat 2.5 ug/ml.| Product Name | RNF14 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RNF14, ARA54, HFB30, TRIAD2, HRIHFB2038 |
| Product description | RNF14 contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins.The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Five alternatively spliced transcript variants encoding two distinct isoforms have been reported.1) Lin, D.Y., (2004) Mol. Cell. Biol. 24 (24), 10529-10541. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RNF14. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| RNF14 antibody can be used for detection of RNF14 by ELISA at 1:312500. RNF14 antibody can be used for detection of RNF14 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RNF14 |
|---|---|
| Alternative Names | RNF14 ARA54 HFB30 TRIAD2 HRIHFB2038 |
| Molecular Weight(MW) | 52 kDa, 54 kDa, 40 kDa, 54 kDa, 54 kDa |
Application
Antibody used in IHC on Human kidney.
Application
Antibody used in WB on Human Jurkat 2.5 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | RNF14 antibody can be used for detection of RNF14 by ELISA at 1:312500. RNF14 antibody can be used for detection of RNF14 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store RNF14 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
