RNF20 shares similarity with BRE1 of S. cerevisiae. Yeast BRE1 is an ubiquitin ligase required for the ubiquitination of histone H2B and the methylation of histone H3. The protein encoded by this gene shares similarity with BRE1 of S. cerevisiae. Yeast BRE1 is a ubiquitin ligase required for the ubiquitination of histone H2B and the methylation of histone H3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.1) Barber, T.D., (2008) Proc. Natl. Acad. Sci. U.S.A. 105 (9), 3443-3448.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RNF20.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
WB
RNF20 antibody can be used for detection of RNF20 by ELISA at 1:62500. RNF20 antibody can be used for detection of RNF20 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
RNF20 antibody can be used for detection of RNF20 by ELISA at 1:62500. RNF20 antibody can be used for detection of RNF20 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store RNF20 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.